Butterworth Lab Department of Cell Biology University of Pittsburgh
Butterworth LabDepartment of Cell BiologyUniversity of Pittsburgh

Publications & Lab Members

Espiritu E.B., Crunk A.E., Bais A., Hochbaum D., Cervino A.S., Phua Y.L., Butterworth M.B., Goto T., Ho J., Hukriede N.A., Cirio M.C. (2018). The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development. Scientific Reports. 8(1):16029. PMID: 30375416


Mukherjee, A., Wang, Z., Kinlough, C.L., Poland, P.A., Marciszyn, A.L., Montalbetti, N., Carattino, M.D., Butterworth, M.B., Kleyman, T.R. and Hughey, R.P. (2017). Specific palmitoyltransferases associate with and activate the Epithelial Sodium Channel. Journal of Biological Chemistry. 292(10):4152-4163



Klemens, C.A, Edinger, R.S, Kightlinger, L., Liu, X. and Butterworth, M.B. (2017). Ankyrin G Expression Regulates Apical Delivery of the Epithelial Sodium Channel (ENaC). J. Biol. Chem. 292(1):375-385.

PMID: 27895120


Liu X, Edinger RS, Klemens CA, Phua YL, Bodnar AJ, LaFramboise WA, Ho J, Butterworth MB. (2016). A MicroRNA Cluster miR-232427 Is Upregulated by Aldosterone in the Distal Kidney Nephron where It Alters Sodium Transport. J Cell Physiol. 232(6):1306-1317.

PMID: 27636893


Li Y, Hu H, Butterworth MB, Tian JB, Zhu MX, O'Neil RG. (2016). Expression of a Diverse Array of Ca2+-Activated K+ Channels (SK1/3, IK1, BK) that Functionally Couple to the Mechanosensitive TRPV4 Channel in the Collecting Duct System of Kidney. PLoS One. 11(5):e0155006

PMID: 27159616


Roy, A., Al-Qusairi, L., Donnelly, B.F., Ronzaud, C., Marciszyn, A., Gong, F., Chang, Y.P, Butterworth, M.B., Pastor-Soler, N., Hallows, K.R., Staub, O. and Subramanya, A.R. (2015). Alternatively spliced proline-rich cassettes link WNK1 to aldosterone-dependent signaling cascades.  Journal of Clinical Investigation. 125(9):3433-48.

PMID: 26241057


Butterworth, M.B., Zhang, L and Thibodeau, P.H. (2014). Modulation of the Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by a Pseudomonas aeruginosa Protease Inhibitor. PLOS One. 9(6): e100313.

PMID: 24963801


Edinger. R.S., Coronnello. C., Bodnar, A.J., LaFramboise, W.A., Benos, P.V., Ho, J., Johnson, J.P and Butterworth, M.B. (2014). Aldosterone regulates microRNAs in the CCD to alter sodium transport. Journal of the American Society of Nephrology. 25 (11):2445-57.


Bertuccio, C.A., Lee, S-L., Wu, G., Butterworth, M.B., Hamilton, K.L. and Devor, D.C. (2014). Anterograde trafficking of KCa3.1 in polarized epithelia is Rab1- and Rab8-dependent and recycling endosome-independent. PLOS One. 9(3):e92013


Edinger, R.S., Bertrand, C.A, Rondandino,C., Apodaca,G.A., Johnson, J.P. and Butterworth, M.B. (2012). The epithelial sodium channel (ENaC) establishes a trafficking vesicle pool responsible for its regulation. PLOS One 7 (9):e46593.


Butterworth, M.B., Zhang L., Heidrich E.M., Myerburg M.M., Thibodeau P.H. (2012). Activation of the epithelial sodium channel (ENaC) by the alkaline protease from Pseudomonas aeruginosa. Journal of Biological Chemistry. 287(39):32556-65.


Butterworth M.B., Edinger R.S., Silvis M.R., Gallo L.I., Liang X., Apodaca G., Frizzell R.A., Johnson J.P. (2012). RAB11b REGULATES THE TRAFFICKING AND RECYCLING OF THE EPITHELIAL SODIUM CHANNEL (ENaC). Am. J. Physiol. Renal Physiol. 302:F581-90.


Myerburg M.M., Harvey P.R., Heidrich E.M., Pilewski J.M., Butterworth M.B. (2010). Acute regulation of ENaC in airway epithelia by proteases and trafficking. American Journal of Respiratory Cell and Molecular Biology. 43(6): 712-9.


Liang, X.; Peters, K.W.; Butterworth, M.B., Frizzell, R.A. (2010). AS160 modulates aldosterone stimulated epithelial sodium channel (ENaC) forward trafficking. Molecular Biology of the Cell. 21(12):2024-2033.


Hallows, K.R.; Edinger, R.S.; Butterworth, M.B.; Oyster, N.M.; Li, H.; Wang, H.; Buck, J.; Levin, L.R.; Johnson, J.P. and Pastor-Soler, N.M. (2009). Novel regulation of epithelial Na+ transport by soluble adenylyl cyclase in kidney collecting duct cells. Journal of Biological Chemistry. 284(9): 5774-83


Silvis, M.R.; Bertrand, C.A.; Ameen, N.; Golin-Bisello, F.; Butterworth, M.B., Frizzell, R.A. and Bradbury, N.A. (2009).  Rab11b regulates the apical recycling of CFTR in polarized intestinal epithelial cells. Molecular Biology of the Cell. 20(8): 2337-50


Liang, X.; Butterworth, M.B. ; Peters, K.W., Walker, W.H. and Frizzell, R.A. (2008). An obligatory heterodimer of 14-3-3 beta and 14-3-3 epsilon is required for aldosterone regulation of the epithelial sodium channel. Journal of Biological Chemistry. 283: 27418-27425


Butterworth, M.B.; Edinger, R.S.; Ovaa, H.; Johnson, J.P. and Frizzell, R.A.  (2007). The deubiquitinating enzyme UCH-L3 regulates the apical membrane recycling of the epithelial sodium channel.  Journal of Biological Chemistry. 282:37885-93


Hill, W.G.; Butterworth, M.B*.; Wang, H. Edinger, R.S.; Frizzell, R.A. and Johnson, J.P. (2007).  Lipid rafts mediate constitutive apical delivery of the epithelial sodium channel (ENaC).  Journal of Biological Chemistry. 282:37402-11 (*Co-first author)


Myerburg, M.M.; Butterworth, M.B.; McKenna, E.; Frizzell, R.A.; Kleyman,T.R. and Pilewski, J.M.(2006).  Airway surface liquid depth regulates ENaC by altering the serine protease-protease inhibitor balance: A mechanism for sodium hyperabsorption in cystic fibrosis. Journal of Biological Chemistry. 281: 27942-49.


Liang, X.; Peters, K.W.; Butterworth, M.B. and Frizzell, R.A. (2006). 14-3-3 isoforms are induced by aldosterone and participate in its regulation of epithelial sodium channels. Journal of Biological Chemistry. 281: 16323-32.


Wang, H.; Traub, L.; Weixel, K.; Shah, N.; Edinger, R.S.; Perry C.; Kleyman T.R.; Butterworth M.B.; Frizzell, R.A. and  Johnson, J.P.  (2006).  Clathrin-associated endocytosis of ENaC: role of epsin. Journal of Biological Chemistry. 281: 14129-35.


Zhang, H. ; Schmidt, B.Z.; Sun, F.; Condliffe, S.B.; Butterworth, M.B.; Youker, R.T.; Brodsky, J.L.; Aridor, M and Raymond A. Frizzell (2006).  Cysteine string protein monitors late steps in CFTR biogenesis.  Journal of Biological Chemistry.  281: 11312-21.


Butterworth, M.B.; Frizzell, R.A.; Johnson, J.P; Peters, K.W. and Edinger, R.S.  (2005).  PKA-dependant ENaC trafficking requires the SNARE binding protein complexin.  American Journal of Physiology-Renal. 285(5):F969-977.


Butterworth, M.B.; Edinger, R.S.; Johnson, J.P and Frizzell, R.A.  (2005). Acute ENaC regulation by cAMP in a kidney cell line is mediated by exocytic insertion from a recycling channel pool.  Journal of General Physiology. 125: 81-101.


Blazer-Yost, B.L.; Butterworth, M.; Hartman, A.; Parker, G.E.; Faletti, C.; Els, W.J. and Rhodes, S.J. (2001). Characterization of A6 cell clones expressing GFP-labeled ENaC subunits.  American Journal of Physiology-Cell Physiol.  281(2):C624-32.


Butterworth, M.B.; Helman, S.I. and Els, W.J.(2000).  cAMP-sensitive endocytic trafficking in A6 epithelia. American Journal of Physiology-Cell Physiol.  280(4): C752-C762.


Els, W.J. and Butterworth, M.B. (1998).  The localisation of adenylate cyclase in membranes of cultured renal cells.  Journal of Microscopy Research & Technique 40:455-462.



Review Articles, Book Chapters and Commentaries


Regulation of aldosterone signaling by microRNAs. Butterworth MB and Alvarez de la Rosa D. In: Aldosterone, Volume 109 1st Edition (in Press, Elsevier 2019)


Butterworth MB.(2018). Role of microRNAs in aldosterone signaling. Curr Opin Nephrol Hypertens. 27 (5):390-394


Butterworth MB.(2018). The Tale of Two (Distal Nephron) Cell Types.  Am J Physiol Renal Physiol. 314 (5):F930-F931.


Butterworth, M.B. (2015). MicroRNAs and the regulation of aldosterone signaling. American Journal of Physiology-Cell Physiol.  308(7):C521-C527.


Thibodeau P.H. and Butterworth M.B. (2012). Proteases, Cystic Fibrosis and the Epithelial Sodium Channel (ENaC). Cell & Tissue Research 351(2):309-23


Butterworth, M.B. (2010). Regulation of the epithelial sodium channel (ENaC) activity by membrane trafficking. Biochim Biophys Acta – Molecular Basis for Disease. 1802 (12): 1166-77


Butterworth, M.B.; Edinger, R.S.; Frizzell, R.A. and Johnson, J.P.  (2009).  Regulation of the epithelial sodium channel (ENaC) by membrane trafficking.  American Journal of Physiology – Renal.  296: F10-F24


Butterworth, M.B; Weisz, O.A. and Johnson, J.P.  (2008).  Some assembly required:  Putting the epithelial sodium channel together.  Journal of Biological Chemistry.  283(51): 35305-9


Butterworth, M.B. and Johnson, J.P  (2008).  USP10 the Nexus between Nexin and Vasopressin. American Journal of Pysiology – Renal.  295(4): F888

Lab Members

Michael Butterworth (PI)

Nejla Ozbaki Yagan (Research Specialist)

Xiaoning Liu (Research Specialist)

Kaylee Williams (Undergraduate Student Worker)


Bailey Cramer (Undergraduate Student Worker)


Department of Cell Biology

S314 SBT (office)

S353/9 (labs)
203 Lothrop St, Pittsburgh, PA

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Michael Butterworth, PhD